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=media type="file" key="Monty Python - Medical Love Song.mp3" width="240" height="20"= toc =Welcome to Biomedical Innovation 2012=

Test your students knowledge: Preassesment
Pipetting skills: You will set up a serial dilution. The students will have to pipette certain volumes into tubes and then plate the final tube. The plate will show growth specific to the concentration of microorganism. Tube one: Water, 200ul Tube two: Water with __#|Ecoli__, 200ul Students measure Tube A: 40ul of tube 1 and 40ul of tube 2 Tube B: 60ul of tube 1 and 20ul of tube 2 Tube C: 50ul of tube 1 and 30ul of tube 2 Tube D: 30ul of tube 1 and 50ul of tube 2 Tube E: 20ul of tube 1 and 60ul of tube 2

Have students plate A-E by pipetting 40ul from each tube and dispensing onto a plate. Let the liquid dry before putting in the incubator. Incubate for 24hrs.









Logger Pro: Have sensor stations and let the students do screenshots of the running of the sensor (like in training).

New things section:
Statistics: Pull from 2.1.1 and 5.1.1 and do stats with your students. Have them review real research __#|studies__ and look at how they applied stats to the work. Examples: Cloning: 6.1.1 challenge starting with plasmid section Plasmid construction: use your creative juices to have students design plasmids physically. Use varying colors to denote different sections of the plasmid (gene of interest, ORI, Selective Marker) Swim noodles and pvc Sting and paperclips Pipe cleaners and beads

[] This website gives APA format fro citations and papers as well as a lit. review check it out!

Emergency Room Problem One
You tube video 2010 excel instructions

I give my students a community. They then have to develop their emergency innovation based on their community. Each community is given a budget to stay within.

Document for __#|Gantt chart__ in excel 2010 Ideas for the classroom: __Five Step Analysis for Triage:__ 16 patients provided in curriculum are a three step triage. Current hospital practices use a five step. Have students compare a 5 step and a three step with the 16 patients. __Presentation Patient Challenge__: During final presentation taking five patients arriving in 15 minute time frame to challenge the emergency room innovation __Bid war__: Medical engineers have to provide bids to hospitals. Have multiple groups assigned to a community then during presentations have a winner declared for that bid. Your __#|hospital administrators__ could be fellow teachers; building administrators; real hospital personnel; or advisory board . Movie ideas for problem one: Episodes of ER, Mystery of ER, Real stories of the ER

Problem 2: Human Physiology
Statistics for biology Teachers using excel: This book is wonderful. Check out the graph on page 24!!!

__Poster website:__ http://people.eku.edu/ritchisong/posterpres.html

__Science and the Media:__ Here are other ideas for science and the media: Have your students pick one of these ideas. Then they can investigate both sides of the story. Have them try to find the research data and analyze the research.(Reinforce the validation of website credentials). Have your students pick a side and defend their side with facts. You should reinforce the idea of subjective and objective data. This is a wonderful tool to introduce the ethics side of science.
 * Gardasil 1**
 * Gardasil 2**
 * Duke lab with cancer research 1**
 * Duke lab with cancer research 2**
 * Vaccines and Autism: community acceptance not scientific acceptance**
 * Vaccine Safety**
 * Adult vaccines**
 * IVF high doses of hormones needed?**
 * Herbal medications**
 * GMO's**
 * __#|Second hand smoke__ and cancer**
 * People are capable of multitasking**

__YOU TUBE video:__ http://www.youtube.com/watch?v=JlfLnx8sh-o

[[file:Excel directions for the example in unit 2.docx]]
__Analysis of variance between groups website:__ You can use this website to help students that are doing studies between similar groups! http://www.physics.csbsju.edu/stats/anova.html

__Why 5% for statistical significance?__ I asked my AP Stats teacher and he said to do this activity (in college a teacher used this activity to explain the 5%). Take a deck of cards and tell your students that the first one to receive a black cards get something. You start passing our the cards and they are all red (the deck will only contain red cards). Once you are done passing out cards or when the students start to really complaining stop, ask the question when did you become skeptical? He said you could do the math here to show the rate of skepticism. It should come out to be 4-5%. You can also have them do the math to show the rate at which a black card should come up after each student. So, based on our own human skepticism that percentage is established.

Movie ideas for problem two: Extraordinary measures

Problem 3: Medical Innovation
__Rubrics for 3.1.2__

__Sample Research log and literature review__ One sheet wonder: If they like charts here is an example:

[[file:Chart Research Studies.docx]]
Tracking of their research: You can use this document to track where they are researching. They should understand that the internet is not the only tool for research. This document helps to promote databases!!

Sales Pitch: Students design a small biotech business around their medical innovation. Here is Carol's Rubric

Movie Ideas for problem three: Faceoff, Dolphin Tale, Millennium Man, Time travelers Wife

Problem 4: Water Contamination
__Our idea to collaborate, consolidate, and challenge our students with water!__

This is the problem that can be collaborated with EDD. You will need to beef it up!! EDD has to design a water filtration system to decrease turbidity using recyclable materials. This is the first problem that they will face in their curriculum. What you can do is use Problem 4 testing procedures and collaborate with EDD to design a water system to be used in third world nations. Your case study can be third world nations contamination rates and disease (www.thewaterproject.org is a wonderful idea source for this project). Have students investigate what are the effects of water contamination in a third world nation. Using 3.1.2 Student Response sheet have EDD and BI do back ground search on the water issues in a third world nation. This should define the constraints and the criteria for the machine. Once the students have completed 3.2.1 have them go through the engineering and design process to develop a water machine to decrease the incidences of sickness due to water contamination. (fyi EDD introduces Project Management and the review of the engineering design process. You can use this to introduce Gantt charts from problem one, presentation components, and potentially poster) BI kids can do an environmental profile of the third world community. This is provided in 4.1.5.

Dose response lab can be applied here (4.1.4). Have students design an experiment to see the effect of different chemicals, like chlorine or UV light response, that can be used to clean water. Then they could use this data to support their machine design. Bacterial dose response lab example. Students would need a plate with colonies. What ever chemical they will test you will do a serial dilution and expose a colony from the plate in a specific dilution. Example: 1%, .8%, .5%, .1% solution (these solution should be in water not broth) one colony from the plate will go into each solution. This should lead the students to then questions their design and how this will be applicable to their machine. Then the students should think about toxicity to humans and the time requirement for the chemical to effectively treat the water. You can test the cell density using a spec or plate your samples. You should do an initial spec or plate once you inoculate and then incubate and spec or plate. Compare the cell densities. You can also use water fleas (daphinia) in a dose response. This would be a good time to introduce the concept of model organisms. In college this was never really explained to me.

Best guess on time frame would be a month! Have no clue since this will be the first year.

Here is a word document containing different nations and an explanation of the water problem that exists. I will use this to help start the Environmental Profile of the region for my students. Instead of the Environmental profile, I am also going to ask them to do a Epidemiology, Geological, Climate data, Socio/Economic, and Government profile. This will help them to really understand the problem that faces the nation. Then they will look at systems. Using all of their information they will design a flowchart showing the relationship between geological, climate, socio/ecomoical, government, and epi reports. This will help them design a machine with a purpose.



__Helpful hints:__ 1. When you get kit 953 grow your slant on plates to confirm growth. If you do not get growth complain to Edvotec!! 953 simplified instructions with a teacher hint on growth of bacteria at the top.



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 * Websites for Environmental Profile Research**

Dose Response Ideas: Many toxicity studies include animal studies. So in tandem with the raddish seed experiment you can perform the experiment on daphnia (water flea). They are very sensitive to environmental contamination.

Floating plastic island!! Incorporates Molecular biology and Medical Innovation: http://www.wired.co.uk/news/archive/2012-07/18/nanobots-recycling-plastic

Movie Ideas for problem four: Erin Brokovich, A Civil Action

Problem 5: Epid
Here are the 13 cases that I use to assess students.



Problem five contains correlation stats. You have all of this data and you are looking for relationships to something based on your research. Here is an idea have your students set up a survey based on something they are interested in. Have them collect information and try doing the stats on their data. Example of this would be the recent Purdue research about abuse and cancer risk. http://www.fox59.com/news/education/wxin-purdue-child-abuse-cancer-child-abuse-can-increase-cancer-risk-purdue-study-says-20120717,0,4533057.column

Here is another study, college grads do not make the grade. Have students assess the questions, individuals surveyed, and the data presented! Should the media have presented this as 49% of college graduates are not ready for the work force? This study was recently repeated by Inside Indiana Business, http://www.insideindianabusiness.com/newsitem.asp?ID=54737



Problem 6: Molecular Biology
Simple lab instructions:

Restriction Enzyme Cheat Sheet

Making Bacterial competent cells: Videos for restriction enzymes and plasmids. []

[|http://wn.com/Recombinant_DNA_Hose_Plasmid#videos]

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Movie Ideas for Problem 6: The island of Dr. Moreau (go for original), Gattaca, Neon Flux, Jack, Benjamin button

Problem 7: Autopsy
Wonderful website for pig dissection:

[[file:pig problems.doc]]


Hint: Try and find a FDA approved facility that could give you pigs, usually for free! Students love it!

Problem 8
As a teacher you should not the boundaries for your class research. You should stay within the BSL1. This means that you will only use minimal protection. When dealing with genetic engineering be very careful. FDA came out with new guidelines dealing with plants! My rule of thumb is if a parent would question when their kid told them about the experiment then permission forms are needed. Example: Mom today at school I had to drink different red liquids and then they tested my heart rate. You may want permission! Use of animals: Pets not lab rats!!

How to make Nanoparticles
These particles will last 6 months. Have students research applications of nanotech. Then have them come up with a way to innovate. From MI they should know about the different structures, have the students figure out the functions of the different structures. __Example:__ Diabetic tattoo

You are not special video:
Watch this video for yourself. I am going to show it to my BI students the first day. And have a discussion on what makes up special and really is it special. What makes us shine as students? Because many of my students are involved in so many different things. When I question why, they state because it looks good on a college application. You are not special commencement speech

Citizens in STEM
The goal of this is to help students realize their role in their global, national, and local community. In problem 5 students look at problems in these three areas and then write a grant for one of their ideas. This will help to promote this idea of "with great power comes great responsibility." What is the responsibility? Jefferson Awards is a program that focuses on community service. They want students to look past self and focus on community. So, wouldn't it be cool to take this and adapt it to STEM. My students will be doing community service but with a STEM relations. This can be modeled by Doctors with out borders or other programs similar to this. The goal is to have students develop independent research that is not self motivated but community motivated. Jefferson Awards is a competition also. Some community ideas: Outreach to 4,5,6th graders: last year I had our EDD and BI kids go to 4,5,6th graders and work with them on a problem. I talked with the teachers and found areas in the curriculum where I designed a problem for them to work on. This teaches problem solving skills and also I wanted to highlight the female involvement in STEM (specifically engineering). After school stuff for kids: Design after school things for kids. This would include: nano, synthetic biology, vex, water testing, or other ideas your students come up with. Have the BI or EDD students lead the activities.